is reconstitution solution the same as bacteriostatic water?

Should I dilute with sterile water (USP grade) or BW? Now, before you start flexing your peptide muscles, you need to learn the proper way to reconstitute them. You may be surprised to learn that they are not interchangeable [5]. Where water is required for preparing or diluting medications for use in neonates, only preservative-free Sterile Water for Injection should be used. Inspect reconstituted (diluted or dissolved) drugs for clarity (if soluble) and freedom from unexpected precipitation or discoloration prior to administration. Do NOT use sterile water for injection for flushing VADs. Bacteriostatic Water (bacteriostatic water for injection) is a highly purified, sterilized water containing 0.9% benzyl alcohol that is used to dilute or dissolve medications to the appropriate consistency for injection. Plus, peptide solutions made with bacteriostatic water resist contamination. What you should be doing is drip the water onto the inner surface of the vial so that there is enough time for the lyophilized peptide and water to mix. The next and immediate step is to add the water you have drawn out into the peptide vial slowly yet smoothly. For more information, please see our Bacteriostatic Water (bacteriostatic water (bacteriostatic water for injection) for injection) for Injection, USP containing additives should be given to a pregnant woman only if clearly needed. Parenteral preparations containing benzyl alcohol should not be used in epidural or spinal anesthesia procedures. First, lets break down the basics of sterile water. You will learn its various applications, how it differs from sterile water, and your best online source to buy bacteriostatic water kits. If you feel that any of our content is inaccurate or out-of-date, please contact us at: [emailprotected]. Validation of these types of filling operations should include media fills and the sampling of critical surfaces and air during active filling (dynamic conditions). Please let me know if you get an answer. On the other hand, distilled water is rather more sterilized and contains neither organic nor organic materials. The most common side effects of H2Oinclude: Tell the doctor if you have any side effect that bothers you or that does not go away. To the extent that Peptides.org references a product that is also a prescription medication, Peptides.org does not does not offer medical diagnosis or treatment advice. Some of the older lyophilizers cannot tolerate steam under pressure and sterilization is marginal at best. See PRECAUTIONS. To answer this question, let's look at an example of Dr. Smith, a pharmaceutical scientist who needs to prepare a peptide solution for a clinical trial. Sterile water is labeled for both injections and irrigations whereas bacteriostatic water is only labeled for injections. Obviously, the batching or compounding of these bulk solutions should be controlled in order to prevent any potential increase in microbiological levels that may occur up to the time that the bulk solutions are filtered (sterilized). For intravenous infusion, first prepare solution by adding not more than 2 mL of Bacteriostatic Water for Injection to the vial; this solution may then be added to 100 . A major difference between the two is the patient population. Where water is required for preparing or diluting medications for use in neonates, only preservative-free Sterile Water for Injection should be used. If an adverse reaction does occur, discontinue the infusion, evaluate the patient, institute appropriate countermeasures, and if possible, retrieve and save the remainder of the unused vehicle for examination. This method can preserve the peptides stability and activity for several months to years, depending on the peptides properties and storage conditions. Haven't had any problems with this bacteriostatic water. Because visual guidance is far better to approach, I searched for some of the Youtube videos that could help you in the process. Reddit and its partners use cookies and similar technologies to provide you with a better experience. 100 mg Plain. Two independent temperature sensing systems. One would be used to control and record temperatures of the cycle as with sterilizers, and the other would be in the cold spot of the chamber. Because most peptides are sold in powder form, one needs to dissolve/dilute them before really using them. Last updated on Dec 1, 2022. Certain areas of this Website are restricted from being access by you and Buckle LLC may further restrict access by you to any areas of this Website, at any time, in absolute discretion. Using either in the proper conditions can provide extraordinary results. Also, there should be provisions to isolate particular sections of the filling operations when low or high fills are encountered. This is the method of peptide reconstitution with bacteriostatic water for injection [13]: Human growth hormone has a range of therapeutic applications, from enhanced physical composition and performance, to treatment of hormone deficiency in adolescents and children [14, 15]. Ob es sich um die Verwaltung von Kundeninvestitionen oder einen umfassenden Plan fr sie zu schaffen und ihre Familie sind unsere zertifizierten Planern gewidmet mit einem auergewhnlichen Service zu bieten. This guide has you covered. Because different stoppering equipment is required, stoppers are sometimes placed on vials by operators rather than by machine. 2) Decide how much reconstitution liquid you want to use to convert your lyophilized peptide into an injection-ready solution, and calculate the concentration of the solution. The pH is 5.7 (4.5 to 7.0). Not only is this inefficient, but you may come across low-quality products from inferior manufacturers. Read more. If both items are the same (benzyl alcohol bacteriostatic solutions) why use the different name? Using in such a matter can cause hemolysis or destruction of red blood cells. The proper reconstitution of HGH before injection closely follows the above method. Our focus is to exclusively link to peer-reviewed studies found on respected websites, like PubMed. 1996-2023 RxList, Inc. An Internet Brands company. When necessary, the pH of each formula was adjusted with sodium hydroxide so that the pH of the reconstituted solution is within the USP specified range of 7 to 8 and the tonicities are, for the 40 mg per mL . The benzyl alcohol in the water makes bacteriostatic water ideal for diluting any drug that will be administered by intramuscular, intravenous, or subcutaneous injection. Bacteriostatic water has a 0.9% benzyl alcohol content and an average pH level of 5.7, while sterile water is free of additives with a pH level of 5.0-7.0. If the product is reconstituted with Sterile Water for Injection, USP, use immediately and discard any unused portion. and discoloration prior to administration, whenever solution and container permit. Do NOT reuse the same saline syringe to flush VADs before and after medication administration. Deus Medicalbelieve in completing the challenges usually faced by pharmaceutical companies in India. Storing peptides is a very crucial step because a slight fluctuation in the physical conditions can really degrade the peptide vial. These Terms will be governed by and interpreted in accordance with the laws of the State of New York, and you submit to the non-exclusive jurisdiction of the state and federal courts located in New York for the resolution of any disputes. 420 Throckmorton Street, Suite 200, Fort Worth Texas 76102, Copyright 2022 BacteriostaticWater.com - All Rights Reserved, Wholesale Hospira Bacteriostatic Water Supplier, Need help? May contain hydrochloric acid for pH adjustment. There is literally no other option. Benzyl alcohol can be a topical irritant but in the concentration of 0.9% does not typically cause irritation and may provide some anesthetic effects. Short-term storage can be done at 4C, while long-term storage can be done by freezing at -20C or -80C, or by lyophilization. Generally lyophilized products have short expiration dates. Carefully install the needle tip on a syringe. Peptides.org makes no claims that any products referenced can cure, treat or prevent any conditions, including any conditions referenced on its website or in print materials. The following preparation is designed solely for parenteral use only after addition of drugs that require dilution or must be dissolved in an aqueous vehicle prior to injection. Both bacteriostatic water and sterile water are not used for straight injections, they have to be diluted with a drug or solvent. Use aseptic technique for single or multiple entry and withdrawal from all containers. However, this method requires special equipment and expertise. Recent inspections have disclosed potency and sterility problems associated with the manufacture and control of lyophilized products. Bacteriostatic Water (bacteriostatic water for injection) is a highly purified, sterilized water containing 0.9% benzyl alcohol that is used to dilute or dissolve medications to the appropriate consistency for injection. It depends on the compound, there is no universal conversion, About: Muscle and Brawn is a coaching company specializing in bodybuilding, hormones, peptides, anabolics, and other biohacking. In case you experience any of these side effects, you should immediately stop using sterile water for injections and talk to a doctor. Water is an essential constituent of all body tissues and accounts for approximately 70% of total body weight. event these should occur, re-evaluate the patient and institute appropriate Archived post. When it comes to reconstituting peptides, there is a common question of whether bacteriostatic water is the same as sterile water. As the recommended dosage is 500mcg, dividing the total of 5000mcg by 500mcg will yield 10 injections per BPC 157 vial. However, if you can get your hands on some, then congratulations, youre already ahead of the game! Follow medical advice and relevant indications for maximum safety and results when using bacteriostatic water for injections. Bacteriostatic water is very easy to manufacture while sterile water is an arduous process. The preservative agent (0.9% benzyl alcohol) in bacteriostatic water allows packaging in multi-dose vials that resist contamination for up to 28 days when used with sterile needles. The action you just performed triggered the security solution. Though their features overlap, bacteriostatic water and sterile water have several significant differences. and our Bacteriostatic Water may be used alone or with other medications. Patients, athletes, healthcare workers and others use sterile solutions to help in medication delivery, injections, irrigation and other uses. Bacteriostatic Water (bacteriostatic water (bacteriostatic water for injection) for injection) for Injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% (9 mg/mL) of benzyl alcohol added as a bacteriostatic preservative. It is essential to avoid repeated freeze-thaw cycles and exposure to light and high temperatures. Typically available as lyophilized powders to maintain purity and integrity, peptides require reconstitution before injection [12]. ZOMACTON 10 mg reconstituted with bacteriostatic water for injection containing 0.33% metacresol should not be used if the patient is allergic to metacresol. Because of the benzyl alcohol contained in Bacteriostatic Water for Injection, USP, when doses greater than . These Terms will be applied fully and affect to your use of this Website. Researchers also use organic solvent such as Acetic acid and DMSO to dissolve hydrophobic peptides and polar uncharged amino acids. Statements regarding products presented on Peptides.org are the opinions of the individuals making them and are not necessarily the same as those of Peptides.org. Lets explore their points of similarity and difference to understand their appropriate uses. Because many of the types of products are antibiotics, subpotency in a vial can present a potentially very serious situation. Typical finished product testing that should be reviewed includes dose uniformity testing, sterility testing, and any required stability testing of aged batches of reconstituted solutions. In case you have any issue diluting peptides in bacteriostatic water, you should add a small amount of 10% aqueous acetic acid for basic peptides and aqueous ammonia for acidic peptides. It could be potentially harmful to try to reconstitute a peptide meant for mixing with bacteriostatic water with sterile water. Those are bacteriostatic and sterile water. 2. : regeneration of an organic form by reorganization of existent tissue without blastema formation. There are several actions that could trigger this block including submitting a certain word or phrase, a SQL command or malformed data. Drugs.com provides accurate and independent information on more than 24,000 prescription drugs, over-the-counter medicines and natural products. Pregnancy: Animal reproduction studies have not been conducted with Bacteriostatic Water for Injection. Now we will explore some of the most exciting applications. Parenteral drug products should be inspected visually for particulate matter The action you just performed triggered the security solution. . Gather the following: alcohol swabs, bacteriostatic water, peptide powder, and a sterile syringe. Please enter the email address for your account. 1) Determine the quantity of peptide, in micrograms (mcg), in your vial. See WARNINGS, PRECAUTIONS and ADVERSE REACTIONS. On the other hand, a sterile water vial doesnt contain any preservatives/additives which can keep it free of pathogens for a longer time, thus one will have to use it immediately after opening the seal. The choice is purely up to the user which solution to use. Intravenous administration of Bacteriostatic Water (bacteriostatic water (bacteriostatic water for injection) for injection) for Injection without a solute may result in hemolysis. Now here comes the crux of the article after talking about some of the major prerequisites of the peptide reconstitution process. If an adverse reaction does occur, discontinue the infusion, evaluate the patient, institute appropriate countermeasures, and if possible, retrieve and save the remainder of the unused vehicle for examination. Bacteriostatic Water for Injection Prescribing Information. Bacteriostatic waters preservative content prevents contamination for multiple doses, while sterile water may be contaminated after one use. The container requires no vapor barrier to maintain the proper labeled volume. Report. Parenteral preparations with benzyl alcohol should not be used for fluid replacement. The semi-rigid vial is fabricated from a specially formulated polyolefin. Contraindications for bacteriostatic water injections include newborn babies and cerebrospinal injections [2, 8]. Water balance is maintained by various regulatory mechanisms. Home > Peptides > Peptide Reconstitution + Mixing Guide with Calculator. The re-use of sterile water for injections is strictly prohibited. No, you cant just inject bacteriostatic water alone! Sterilization procedures should parallel that of an autoclave and a typical system should include: Provisions for sterilizing the shelf support rods unless they are only exposed to the inside of the chamber after stoppering. Sorry Red, I was ambiguous with my 1st post. It may be wise to always opt for bacteriostatic water if both water types are allowed for use. The filling of vials that are to be lyophilized has some problems that are somewhat unique. Sample 1 Sample 2 Sample 3. Step 1: Wash your hands and gather your materials. Bacteriostatic water solutions remain sterile and safe for longer due to the preservative agent. Due to the lack of antimicrobials, sterile water can easily be contaminated once exposed to surroundings and even yourself. Any transfer and handling, such as loading of the lyophilizer, should take place under primary barriers, such as laminar flow hoods under which the vials were filled. 4 people found this helpful. The small volume of fluid provided by Bacteriostatic Water for Injection, USP when used only as a pharmaceutic aid for diluting or dissolving drugs for parenteral injection, is unlikely to exert a significant effect on fluid balance except possibly in very small infants. They both are USP meaning they have an official monograph or documentation by the United States Pharmacopeia, both are not used for IV solutions. If kept under appropriate conditions, bacteriostatic saline can last up to 28 days. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. Benzyl alcohol, a preservative in Bacteriostatic Water for Injection, USP has been associated with toxicity in neonates. Administration of an estimated 9 mL to a 6 kg infant or neonate is potentially capable of producing blood pressure changes. All Right Reserved. Bacteriostatic Water for Injection may kill some of the vegetative cells if present as contaminants. May contain hydrochloric acid for pH adjustment. No more confusion or frustration, just pure peptide power! (Isotonic saline = 0.28 osmolar.) Withdraw approximately 1mL of bacteriostatic water from the vial and slowly transfer it into the peptide container. As with any ethylene oxide treatment, humidification is necessary. For the reconstitution of HGH powder, bacteriostatic water is a great choice, as it preserves HGH solutions and efficiently dissolves lyophilized aggregates [16]. Bacteriostatic water works well as a peptide solvent, reconstituting the compounds without compromising their effectiveness. 5.161.53.20 The safety of the plastic has been confirmed by tests in animals according to USP biological standards for plastic containers. Although both of these types of water are sterile, the difference between bacteriostatic water and sterile water is that other sterile waters for injection do not contain a bacteriostatic agent. Do not use unless the solution is clear and seal intact. We strive to never have a piece of inaccurate information on this website. Available for Android and iOS devices. Additionally, bacteriostatic water must always be mixed with a solute before injection. Before digging deeper into precise conditions required to store peptides, you should know that keeping a peptide vial away from intense light, high temperature and moisture is a MUST. Overall, specialists agree that bacteriostatic water for injection is safer and more effective in most cases. If symptoms appear, injections should be discontinued pending medical consultation [9]. See WARNINGS, PRECAUTIONS Average normal adult daily requirement ranges from two to three liters (1.0 to 1.5 liters each for insensible water loss by perspiration and urine production). All rights reserved. As it cannot transfer pathogens, it is used mainly used in the medical field. How does the math work when reconstituting a peptide with 5mg of two different peptides in the same vial? The concern with any microbiological level is the possible increase in endotoxins that may develop. It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. In order to reconstitute 10 equal dosages, we need to mix 1ml of bacteriostatic water in all the 10 injections, which means 0.1ml of water per dosage. He is responsible for ensuring the quality of the information presented on our website. However, they do differ in their composition as the bacteriostatic water contains 0.9% benzyl alcohol as a preservative. In the You should never inject bacteriostatic water without mixing in any medications because the benzyl alcohol in it causes the red blood cell lysis. Bacteriostatic Water for Injection, USP must be made approximately isotonic prior to use. Once you have received the verification code, you will be able to choose a new password for your account. This website is using a security service to protect itself from online attacks. It is simply water that has gone through purification and distillation processes for its safe use in various therapeutic contexts, namely injections, dilution, and irrigation. HCG solutions are to be refrigerated to prevent degradation [19]. As defined in Section 10.01. 3977). Click to reveal In summary, the best way to store reconstituted peptides depends on the duration of storage and the desired shelf life. Your IP: Peptide Dosing Guide. Often available as a lyophilized powder, hCG is best reconstituted with bacteriostatic water for injection due to the solvents efficiency and preservative agents. Buckle LLC, including its officers, directors and employees shall not be held liable for any indirect, consequential or special liability arising out of or in any way related to your use of this Website. All you have to do is add the desirable reconstituted peptides in 1 vial and then use the syringe to pull out the mixture so that you can administer it. Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page. Each milliliter (mL) contains sodium chloride 9 mg and 0.9% (9 mg/mL) benzyl alcohol added as a bacteriostatic preservative. But, if there is an absolute need to stock solution form, what I would recommend is to store the required dosage into individual vials. This significantly reduces the risk of infection [, It is an efficient solvent and presents minimal interaction with most medications [, Apart from medications, bacteriostatic water is also indicated for the reconstitution and injection of therapeutic research peptides and hormones [, Suitable injection routes are determined by the type of solute, the recipients needs, and target rates of diffusion and metabolization. Wir verstehen die Bedeutung der Kunden auf dem richtigen Weg zum Erfolg bringen, und wir sind bereit, mit ihnen der Planung in allen Bereichen zu arbeiten. Water distribution depends primarily on the concentration of electrolytes in the body compartments and sodium (Na+) plays a major role in maintaining physiologic equilibrium. While most of the peptides will be soluble in bacteriostatic water and sterile water, some really wont, and this will be depending on the polarity of the peptides. Bacteriostatic 0.9% Sodium Chloride Injection, USP is a sterile, nonpyrogenic, isotonic solution of sodium chloride in water for injection. Allow the powder to naturally dissolve until the vial is transparent. Both may be used in the injection of peptides, hormones, and various medications, often reconstituted from lyophilized powder. The pH is 5.7 (4.5 to 7.0). Buckle LLC is permitted to revise these Terms at any time as it sees fit, and by using this Website you are expected to review these Terms on a regular basis. Insert your needle tip into the sealed bottle of bacteriostatic water. My recommendation is to use bacteriostatic water as that's what most peptide vendors sell. So it all differs and the exact dosage can only be prescribed by your health practitioner. Injection of HGH is considered safe when prescribed by a doctor. FDA indications vary regarding drug interactions. 4,7 For example, daptomycin RF reconstituted in the vial with bacteriostatic water or sterile water and subsequently refrigerated has an in-use storage period of 3 days, and daptomycin RF reconstituted in the vial . Due to the potential toxicity of benzyl alcohol in neonates, solutions containing benzyl alcohol must not be used in this patient population. Bacteriostatic Water for Injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% (9 mg/mL) or 1.1% (11 mg/mL) benzyl alcohol added as a bacteriostatic preservative. ; New York City, Nov. 13, 1968. A verification code will be sent to you. For example, the recommended dosage of BPC-157 is 1mcg to 10mcg per kg of body weight which accounts for 200mcg to 1000mcg per injection. On the other hand, if the peptide vial has been reconstituted, make sure that you store it between 2 and 8 degrees celsius because the reconstituted peptides are susceptible to minor temperature changes. ITG SUBJECT: LYOPHILIZATION OF PARENTERALS. If any provision of these Terms is found to be invalid under any applicable law, such provisions shall be deleted without affecting the remaining provisions herein. By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. May contain hydrochloric acid for pH adjustment. With our easy-to-use reconstitution calculator, you now dont have to worry about all the multiplication, division or the unit conversion and all. Exogenous formulations are available in powder and liquid forms. Performance & security by Cloudflare. Peptide Reconstitution + Mixing Guide with Calculator, Read the full review on BPC 157 only at Muscle and Brawn, Limitless Life Nootropics (and Peptides) Review, Epitalon Peptide Guide: Benefits, Uses, Dosage, How to Safely Use SARMs to Sculpt Three-Dimensional Muscles and Torch Fat, The ONE Thing You MUST do Before starting SARMs if You Want to Keep Yourself Out of the Hospital, The MILDEST SARM that gives you gains without all the bloating, How to Properly Use SARMs to build 3D Muscles Without Experiencing Sloppy Noodle Syndrome. Additionally, this stability testing should include least concentrated as well as the most concentrated reconstituted solutions. This bacteriostat is usually 0.9% benzyl alcohol. The starter set has all you need to get going, including the following: If you want a little more, check out the premium kit: Qualified handlers are sure to enjoy this top online source for research materials. The dosage and administration schedule should follow applicable recommendations [18, 19]. The absence of a bacteriostat in sterile water only allows for a one time use. The first step is to use the alcohol swab and wipe the vials containing lyophilized peptides and the bacteriostatic/sterile water. 1. : the action of reconstituting or state of being reconstituted. You must not use this Website if you disagree with any of these Website Standard Terms and Conditions. Its like a chemistry experiment, but instead of blowing things up, youll be improving your health. IMPORTANT Use only the accompanying diluent or Bacteriostatic Water For Injection with. I have this same question. In no event shall Buckle LLC, nor any of its officers, directors and employees, shall be held liable for anything arising out of or in any way connected with your use of this website whether such liability is under contract. Differences Between Bacteriostatic Water vs. Sterile Water To really know the best solvent in which a peptide will be soluble, you must have to consider some biochemistry knowledge and do some trial and error. Bacteriostatic Water vs reconstitution solution whats the difference? When you buy through links on our site, we may earn a commission. Nothing really. After that, collect and prep your materials. For these types of products, bioburden should be minimal and the bioburden should be determined prior to sterilization of these bulk solutions prior to filling. Use only as a diluent or solvent. You hereby indemnify to the fullest extent Buckle LLC from and against any and/or all liabilities, costs, demands, causes of action, damages and expenses arising in any way related to your breach of any of the provisions of these Terms. Bacteriostatic water and sterile water are essentially used for the same function which is to dissolve the drugs/medications for injecting purposes. With the presence of 0.9% benzyl alcohol, the shelf-life of a bacteriostatic water vial is considerably increased up to 28 days as it will prevent the growth of any bacteria. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. Complement your research with this USP solvent, the go-to solution of the medical and scientific community. This parenteral preparation is unlikely to Two sterile solutions that are commonly used are bacteriostatic water and sterile water. New comments cannot be posted and votes cannot be cast. It also is prepared with a bacteriostat, a compound used to prevent any bacterial growth. The clinical applications of the hormone hCG include fertility enhancement in both women and men, as well as a supplement to TRT in male subjects [17, 18].

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